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(A) Time course kinetics of PCNA complex formation induced by BLM treatment in normal and AT cells. The cells were treated with 10 mU/ml of BLM for 30 min and incubated for various recovery times. The insoluble proteins isolated from control and treated cells were electrophoresed and transferred to PVDF membrane. The membrane was reacted with antibodies to PCNA, p53 and actin. (B) Histogram showing the time course kinetics of PCNA complex in normal and AT cells.
