Table 1. Fluorescence intensity of PCNA in control and γ-ray irradiated NHDF cells.
Treatment | Fluorescence intensity (arbitrary units) |
Control (no extraction) | 80.24 ± 8.50 |
5 Gy (no extraction) | 77.17 ± 7.13 |
Control (buffer I extracted) | 23.35 ± 1.60 |
5 Gy (buffer I extracted) | 83.50 ± 5.50 |
Control (buffer II extracted) | 18.86 ± 1.00 |
5 Gy (buffer II extracted) | 93.30 ± 7.20 |
Images of 15–20 randomly chosen NHDF cells were captured using a Zeiss epifluorescence microscope equipped with a charged coupled device camera and the relative fluorescence intensity of PCNA in control and γ-ray treated cells was measured using Adobe Photoshop and expressed in arbitrary units as mean ± SE.