Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant features | Source or reference |
|---|---|---|
| E. coli | ||
| DH5⟨ | High-efficiency transformation | Bethesda Research Laboratories |
| SM10 | Conjugation proficient | Simon et al. (1983) |
| B. pertussis | ||
| Tohama I | Patient isolate | Kasuga et al. (1954) |
| BP536 | StrR, NalR Tohama I | Stibitz and Yang (1991) |
| BP953 | BP536, fha-lacZ, ptx-phoA | Stibitz (1994) |
| BP1526 | BP953, ΔbvgA–1215 | This study |
| Plasm ids | ||
| pSS3110 | lacZYA promoter assay vector | Veal-Carr and Stibitz (2005) |
| pSS3967 | luxCDABE promoter assay vector | This study |
| pQC1025 | pSS3967::Pfim2* | This study |
| pSS4159 | pSS3967::Pfim3** | This study |
| pQC1030_ | pSS3967::PfimX*** | This study |
| pPuvsX-sigma | T4 uvsX promoter transcription vector | Hinton and Vuthoori (2000) |
| pPre# | PPre# transcription vector | This study |
| pTE103 | in vitro transcription vector | Elliott and Geiduscheck (1984) |
| pfha | pTE103::Pfha | Boucher et al. (1997) |
| pPfim3-13C | pTE103::Pfim3-13C | This study |
| pPfim3-15C | pTE103::Pfim3-15C | This study |
| pETsigmaBp |
B. pertussis  subunit expression |
This study |
*
cloned fim2 fragment contains nucleotides 1166483 to 1176644 of the B. pertussis Tohama I genome and extends, relative to the start of transcription, from −130 to +32.
**
cloned fim3 fragment contains nucleotides 1647430 to 1647592 of the B. pertussis Tohama I genome and extends, relative to the start of transcription, from −130 to +33.
***
cloned fimX fragment contains nucleotides 2839614-2839776 of the B. pertussis Tohama I genome and extends, relative to the start of transcription, from −123 to +40.