Table 1.
Retention of various sulfonylurea drugs on columns containing normal or glycated HSA
| Drug/protein | Retention factor, k | Normalized retention factor, k/(protein content) |
|---|---|---|
| Normal HSA | ||
| Acetohexamide | 4.7 (± 0.4) | 0.19 (± 0.02) |
| Glybenclamide | 1.05 (± 0.07) | 0.042 (± 0.005) |
| Gliclazide | 2.5 (± 0.1) | 0.099 (± 0.011) |
| Tolbutamide | 37 (± 2) | 1.49 (± 0.15) |
| Glycated HSA | ||
| Acetohexamide | 8.6 (± 0.2) | 0.56 (± 0.03) |
| Glybenclamide | 2.3 (± 0.1) | 0.15 (± 0.01) |
| Gliclazide | 4.4 (± 0.1) | 0.28 (± 0.02) |
| Tolbutamide | 61 (± 4) | 4.0 (± 0.3) |
a
All of these studies were conducted at 37°C in the presence of pH 7.4, 67 mM potassium phosphate buffer. The values in parentheses represent a range of ± 1 S.D.
b
These normalized retention factors were calculated by dividing the listed retention factors by a measured protein content for the given supports of 25 (± 2.5) mg HSA per g silica or 15.4 (± 0.7) mg G-HSA per g silica.