Table 2.

Oligonucleotide primers used in this study

Gene	Primer	Sequence	Amplicon size (bp)	Reference
ssrRNA	F-H11	5-CATCCGGTCGATCCTGCC-3	292	19
	R-RH4	5-AGTCGAACCCTGATTCTCCGCCCAGG-3
384-bp β-giardin	F-376	5-CATAACGACGCCATCGCGGCTCTCAGGAA-3	384	19
	R-G759	5-GAGGCCGCCCTGGATCTTCGAGACGAC-3
753-bp β-giardin	F-G7	5-AAGCCCGACGACCTCACCCGCAGTGC-3	753	19
	R-G759	5-GAGGCCGCCCTGGATCTTCGAGACGAC-3
gdh-primary PCR	F-gdhe	5-TCAACGTYAAYCGYGGYTTCCGT-3
	R-gdhi	5-GTTRTCCTTGCACATCTCC-3
gdh-secondary PCR	F-gdhi	5-CAGTACAACTCYGCTCTCGG-3	432	20
	R-gdhe	5-GTTRTCCTTGCACATCTCC-3
tpi-A	F-tpiA	5-GGAGACCGACGAGCAAAGC-3	148	21
	R-tpiA	5-CTTGCCAAGCGCCTCAA-3
tpi-B	F-tpiB	5-AATAGCAGCACARAACGTGTATCTG-3’	81	21
	R-tpiB	5-CCCATGTCCAGCAGCATCT-3

PCR=Polymerase chain reaction