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. 2010 Aug 31;11(4):573–586. doi: 10.1007/s10162-010-0231-6

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© The Author(s) 2010

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FIG. 8 — Immunoprecipitation of otoferlin from bullfrog saccule and subcellular localization in isolated bullfrog saccular hair cells. A Silver stain of a SDS-PAGE gel containing proteins immunoprecipitated from bullfrog sacculus with the anti-otoferlin antibody (HCS-1) or an irrelevant isotype-matched control antibody (anti-V5 antibody). Immunoglobulin heavy chain is observed at approximately 50 kDa. Molecular mass markers in kilodaltons are indicated to the right. B Confocal localization of actin (red), otoferlin (HCS-1 antibody, green), and PMCAb (blue) in an isolated saccular hair cell by confocal microscopy. Individual channels are shown to the left, merged image is shown to the right. Scale bar = 5 μm.