Confocal images showing colocalization of anterogradely labeled CN-commissural terminal endings with VGLUT2 immunoreactivity in the shell region. Image a and b (40×) are from one animal with injection site shown in inset of a; image c (60×) and d (100X) are from another animal with injection site shown in inset of d. a, b Large (arrows) and small (arrowheads) CN-commissural terminal endings colabeled with VGLUT2 immunoreactivity in the shell region. c, d CN-commissural terminals in shell region (arrow), but not in magnocellular region of VCN, colabeled with VGLUT2 immunoreactivity. Red, VGLUT2 immunoreactivity; green, CN-commissural labeling; yellow, double-labeled terminals. Scale bars = 50 μm (a and b) and 20 μm (c and d).