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. 2010 Jun 24;11(4):675–687. doi: 10.1007/s10162-010-0224-5

FIG. 4.

FIG. 4.

Confocal images (40×) of a retrogradely labeled CN-commissural neuron in the DCN3 showing negative glycine immunoreactivity and negative GABA immunoreactivity. a The retrogradely labeled cell in the DCN3 following injections of FG in the contralateral CN. The inset shows the location of the labeled cell. The straight line indicates the boundary between DCN and inferior cerebellar peduncle (icp). This cell shows both negative glycine immunoreactivity (b) and negative GABA immunoreactivity (c). Confocal multichannel image (overlap of images of a, b, and c) shows no colocalization of FG with either glycine or GABA immunoreactivity (d). Scale bar = 50 µm. DCN3 deep layer of DCN, DAS dorsal acoustic stria, ICP inferior cerebellar peduncle.