Figure 3.
Rec8 cleavage triggers chiasmata resolution and sister centromere disjunction of bivalent chromosomes. (A) Wild-type and Rec8TEV/TEV oocytes expressing H2B-mCherry, Securin-EGFP, and Flag-Mad2 were cultured for 14–16 h. Metaphase I-arrested oocytes were injected with TEV protease mRNA (time 0), and chromosome movements were visualized by time-lapse confocal microscopy (h:mm). Still images from a representative movie of each genotype are shown. The left panels show the H2B-mCherry channel and the right panels show H2B-mCherry and Securin-EGFP pseudocolored in red and green, respectively. Bar, 10 μm. (B) Wild-type and Rec8TEV/TEV oocytes expressing TEV protease were matured in vitro for 5 h. Chromosome spreads were prepared and stained with Hoechst to visualize DNA (red) and CREST to mark centromeres (green).