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. 2001 Mar 15;29(6):e31. doi: 10.1093/nar/29.6.e31

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(A) α1 RNA specifically inhibits the amplification of α1 from a rat kidney cDNA template. PCR was performed with 1 µM sense and 1 µM antisense degenerate primers and rat kidney cDNA; without RNA (lane 1), with 50 nM α1 RNA (lane 2), with 50 nM β1 RNA (lane 3), with 50 nM β2 RNA (lane 4), with 50 nM α1 RNA and 50 nM β1 RNA (lane 5), with 50 nM α1 RNA and 50 nM β2 RNA (lane 6), with 50 nM β1 RNA and 50 nM β2 RNA (lane 7) or with 50 nM α1 RNA, 50 nM β1 RNA and 50 nM β2 RNA (lane 8). After electrophoresis and Southern blotting, the blot was hybridized with a radiolabeled probe specific for α1, washed at high stringency and subjected to autoradiography. A ladder of molecular weight markers is on the left and the arrow indicates the bands corresponding to the expected 750 bp PCR product. (B) β1 RNA specifically inhibits the amplification of β1 from a rat kidney cDNA template. PCR was performed with 1 µM sense and 1 µM antisense degenerate primers and rat kidney cDNA; without RNA (lane 1), with 50 nM α1 RNA (lane 2), with 50 nM β1 RNA (lane 3), with 50 nM β2 RNA (lane 4), with 50 nM α1 RNA and 50 nM β1 RNA (lane 5), with 50 nM α1 RNA and 50 nM β2 RNA (lane 6), with 50 nM β1 RNA and 50 nM β2 RNA (lane 7) or with 50 nM α1 RNA, 50 nM β1 RNA and 50 nM β2 RNA (lane 8). After electrophoresis and Southern blotting, the blot was hybridized with a radiolabeled probe specific for β1, washed at high stringency and subjected to autoradiography. A ladder of molecular weight markers is on the left and the arrow indicates the bands corresponding to the expected 750 bp PCR product. (C) β2 RNA specifically inhibits the amplification of β2 from a rat kidney cDNA template. PCR was performed with 1 µM sense and 1 µM antisense degenerate primers and rat kidney cDNA; without RNA (lane 1), with 50 nM α1 RNA (lane 2), with 50 nM β1 RNA (lane 3), with 50 nM β2 RNA (lane 4), with 50 nM α1 RNA and 50 nM β1 RNA (lane 5), with 50 nM α1 RNA and 50 nM β2 RNA (lane 6), with 50 nM β1 RNA and 50 nM β2 RNA (lane 7) or with 50 nM α1 RNA, 50 nM β1 RNA and 50 nM β2 RNA (lane 8). After electrophoresis and Southern blotting, the blot was hybridized with a radiolabeled probe specific for β2, washed at high stringency and subjected to autoradiography. A ladder of molecular weight markers is on the left and the arrow indicates the bands corresponding to the expected 750 bp PCR product.

(A) α1 RNA specifically inhibits the amplification of α1 from a rat kidney cDNA template. PCR was performed with 1 µM sense and 1 µM antisense degenerate primers and rat kidney cDNA; without RNA (lane 1), with 50 nM α1 RNA (lane 2), with 50 nM β1 RNA (lane 3), with 50 nM β2 RNA (lane 4), with 50 nM α1 RNA and 50 nM β1 RNA (lane 5), with 50 nM α1 RNA and 50 nM β2 RNA (lane 6), with 50 nM β1 RNA and 50 nM β2 RNA (lane 7) or with 50 nM α1 RNA, 50 nM β1 RNA and 50 nM β2 RNA (lane 8). After electrophoresis and Southern blotting, the blot was hybridized with a radiolabeled probe specific for α1, washed at high stringency and subjected to autoradiography. A ladder of molecular weight markers is on the left and the arrow indicates the bands corresponding to the expected 750 bp PCR product. (B) β1 RNA specifically inhibits the amplification of β1 from a rat kidney cDNA template. PCR was performed with 1 µM sense and 1 µM antisense degenerate primers and rat kidney cDNA; without RNA (lane 1), with 50 nM α1 RNA (lane 2), with 50 nM β1 RNA (lane 3), with 50 nM β2 RNA (lane 4), with 50 nM α1 RNA and 50 nM β1 RNA (lane 5), with 50 nM α1 RNA and 50 nM β2 RNA (lane 6), with 50 nM β1 RNA and 50 nM β2 RNA (lane 7) or with 50 nM α1 RNA, 50 nM β1 RNA and 50 nM β2 RNA (lane 8). After electrophoresis and Southern blotting, the blot was hybridized with a radiolabeled probe specific for β1, washed at high stringency and subjected to autoradiography. A ladder of molecular weight markers is on the left and the arrow indicates the bands corresponding to the expected 750 bp PCR product. (C) β2 RNA specifically inhibits the amplification of β2 from a rat kidney cDNA template. PCR was performed with 1 µM sense and 1 µM antisense degenerate primers and rat kidney cDNA; without RNA (lane 1), with 50 nM α1 RNA (lane 2), with 50 nM β1 RNA (lane 3), with 50 nM β2 RNA (lane 4), with 50 nM α1 RNA and 50 nM β1 RNA (lane 5), with 50 nM α1 RNA and 50 nM β2 RNA (lane 6), with 50 nM β1 RNA and 50 nM β2 RNA (lane 7) or with 50 nM α1 RNA, 50 nM β1 RNA and 50 nM β2 RNA (lane 8). After electrophoresis and Southern blotting, the blot was hybridized with a radiolabeled probe specific for β2, washed at high stringency and subjected to autoradiography. A ladder of molecular weight markers is on the left and the arrow indicates the bands corresponding to the expected 750 bp PCR product.