Figure 2. Inclusion of the caveolin-1 scaffolding domain peptide in the pipette-filling solution reduces whole-cell K_ATP current in aortic smooth muscle cells.

A and B, whole-cell current recorded from single isolated rat aortic smooth muscle cells at −60 mV under control conditions (A) or with the inclusion of the caveolin-1 scaffolding domain peptide (SDP; 10 μm) in the pipette-filling solution (B). The arrow indicates the extracellular solution was changed from 6 mm to 140 mm K⁺ to increase the inward driving force for K⁺. C, mean amplitude of glibenclamide-sensitive current in response to inclusion of caveolin-1 scaffolding domain peptide (SDP; 10 μm) or a scrambled version of this peptide (10 μm) in the pipette-filling solution (means ±s.e.m., n= 6, 6, 4 cells, *P < 0.05; n.s., not significant). The caveolin-1 SDP or the scrambled peptide was allowed to dialyse into the cell for 10 min prior to the start of recording.