Figure 4.

Overexpression of wild-type EIG121, but not of EIG121ΔTM and LacZ, induces cytoplasmic vacuolation. (a) T-Rex-293-EIG121 clone A cells, MDA-231-t-EIG121, or MDA-231-t-LacZ cells were grown on coverslips, and tetracycline was added for 0 or 26 h. Cells were then fixed and stained with H&E. (b) Plasmids expressing wild-type EIG121-V5, EIG121ΔTM-V5, EIG121ΔM6PR-V5, or LacZ-V5 fusion proteins were transfected into Ishikawa H cells, and 48 h later, the cells were fixed and stained using an antibody against V5