Figure 8.

EIG121 knockdown compromises starvation-induced autophagy. (a) EIG121 siRNA blocked starvation-induced LC3 degradation. MCF-7 cells were transfected with control nontargeting siRNA or EIG121 siRNA for 72 h and then starved in HBSS for 2 h. Cells were then fixed and stained for LC3. (b) MCF-7 cells were transfected with siRNAs in duplicate and starved as in A, but cell lysates were harvested and probed with an LC3 or EIG121 antibody using western blotting. A representative of one of the three independent experiments is shown. The densities of lanes relative to the first lane of nonstarved cells are shown below the blots. Abbreviations: non, nontransfected cells (no siRNA); EIG, EIG121 siRNA; con, control nontargeting siRNA