. Author manuscript; available in PMC: 2011 Sep 1.

Published in final edited form as: Future Virol. 2010 Sep 1;5(5):593–606. doi: 10.2217/fvl.10.39

Table 1.

Known cleavage sites of NS3 protease in the flaviviral polyprotein precursor.

	Capsid C	NS2A/NS2B	NS2B–NS3	NS3/NS4A	NS4B/NS5
WNV	Q¹⁰¹KKR↓GGTA¹⁰⁸	N¹³⁶⁷RKR↓GWPA¹³⁷⁴	Y¹⁴⁹⁸TKR↓GGVL¹⁵⁰⁵	S²¹¹⁷GKR↓SQIG²¹²⁴	G²⁵²²LKR↓GGAK²⁵²⁹
JEV	Q¹⁰²NKR↓GGNE¹⁰⁹	N¹³⁷⁰KKR↓GWPA¹³⁷⁷	T¹⁵⁰¹TKR↓GGVF¹⁵⁰⁸	A²¹²⁰GKR↓SAVS²¹²⁷	S²⁵⁶⁴LKR↓GRPG²⁵⁷¹
YFV	R⁹⁸ KRR↓SHDV¹⁰⁵	F¹³⁵¹GRR↓SIPV¹³⁵⁸	G¹⁴⁸¹ARR↓SGDV¹⁴⁸⁸	E²¹⁰⁴GRR↓GAAE²¹¹¹	T²⁵⁰³GRR↓GSAN²⁵¹⁰
DV1	R⁹⁷ RKR↓SVTM¹⁰⁴	W¹³⁴¹GRK↓SWPL¹³⁴⁸	K¹⁴⁷¹KQR↓SGVL¹⁴⁷⁸	A²⁰⁹⁰GRR↓SVSG²⁰⁹⁷	G²⁴⁸⁹GRR↓GTGA²⁴⁹⁶
DV2	R⁹⁷ RRR↓TAGV¹⁰⁴	S¹³⁴²KKR↓SWPL¹³⁴⁹	K¹⁴⁷²KQR↓AGVL¹⁴⁷⁹	A²⁰⁹⁰GRK↓SLTL²⁰⁹⁷	N²⁴⁸⁸TRR↓GTGN²⁴⁹⁵
DV3	K⁹⁷ RKK↓TSLC¹⁰⁴	L¹³⁴⁰KRR↓SWPL¹³⁴⁷	Q¹⁴⁷⁰TQR↓SGVL¹⁴⁷⁷	A²⁰⁸⁹GRK↓SIAL²⁰⁹⁶	T²⁴⁸⁷GKR↓GTGS²⁴⁹⁴
DV4	G⁹⁶ RKR↓STIT¹⁰³	A¹³⁴¹SRR↓SWPL¹³⁴⁸	K¹⁴⁷¹TQR↓SGAL¹⁴⁷⁸	S²⁰⁸⁹GRK↓SITL²⁰⁹⁶	T²⁴⁸⁴PRR↓GTGT²⁴⁹¹

The natural cleavage sites exist in the capsid protein C and at the NS2A/NS2B, NS2B/NS3, NS3/NS4A and NS4B/NS5 boundaries of the polyprotein precursor.

The P1′ and P2′ Gly residues are in bold. Arrows indicates the scissile bond.

DV: Dengue virus; JEV: Japanese encephalitis virus; WNV: West Nile virus; YFV: Yellow fever virus.