Table 2.
Primer sequences (5’ to 3’) for quantitative real-time PCR.
| Gene | Forward | Reverse | Efficiency |
|---|---|---|---|
| β-Actin | CTCTTCCAGCCTTCCTTCCT | GGGCAGTGATCTCTTTCTGC | 0.85 |
| ACC | AGCTGAATTTTCGCAGCAAT | GGTTTTCTCCCCAGGAAAAG | 1.07 |
| C/EBPα | TGGACAAGAACAGCAACGAG | GGTCATTGTCACTGGTCAGC | 0.95 |
| FASN | GCATCGCTGGCTACTCCTAC | GTGTAGGCCATCACGAAGGT | 0.93 |
| GAPDH | GGGTCATCATCTCTGCACCT | GGTCATAAGTCCCTCCACGA | 0.93 |
| LPL | GGGTTTTGAGCAAGGGTACA | GCCACAATGACCTTTCCAGT | 0.97 |
| NF-Y | ATGCAGGATCCAAATCAAGC | AAAAGGGCAGAATGTGATCG | 1.05 |
| PPARγ | AGGATGGGGTCCTCATATCC | GCGTTGAACTTCACAGCAAA | 0.86 |
| SCD | TTATTCCGTTATGCCCTTGG | GGTAGTTGTGGAAGCCCTCA | 0.95 |
| Spot14 | CCTCACCCATCTTACCCTGA | CAAGCTAGCAAACTGCACCA | 1.05 |
| SREBP1 | CTGGAGAAGCTGGACTGAGG | GCTTTCCCAAGACTCAGCAC | 0.86 |
| STAT5 | TGGGAAAGATGGGAACTGAG | ACCAACAAGTCTGGGTCAGG | 1.00 |