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. 2010 Jun 28;59(8):1037–1045. doi: 10.1136/gut.2009.199794

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© 2010, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

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Effect of co-culture of gastric epithelial cells with Helicobacter pylori strain 60190 on heparin-binding epidermal growth factor (HB-EGF) expression. (a) HB-EGF gene expression following co-culture of gastric epithelial cells with H pylori pathogenic (60190) and non-pathogenic (Tx30a) strains; *p<0.001 (n=3 replicates per condition and a representative graph is shown, error bars indicate 95% confidence). (b) Cell-associated HB-EGF expression following co-culture of gastric epithelial cells with H pylori pathogenic (60190) and non-pathogenic (Tx30a) strains. Cells were stained for HB-EGF (red fluorescence) and nuclei were counterstained with Hoechst (blue fluorescence); (i) AGS, (ii) AGS/H pylori 60190, (iii) AGS/H pylori Tx30a. Magnification ×20. (c) HB-EGF protein expression level quantified by image analysis following co-culture of gastric epithelial cells with H pylori pathogenic (60190) and non-pathogenic (Tx30a) strains; *significantly higher HB-EGF protein expression level compared with untreated control cells (p<0.03). (d) HB-EGF shedding level following co-culture of gastric epithelial cells with H pylori pathogenic (60190) and non-pathogenic (Tx30a) strains; *significantly higher HB-EGF shedding level compared with untreated control cells (strain 60190 treated: p<0.01 in all cell lines; strain Tx30a treated: p<0.05 in AGS and ST16); #significantly higher HB-EGF shedding level compared with Tx30a treated (p<0.05 in AGS and ST16). (n=3 replicates per condition and a representative graph is shown, error bars indicate 95% confidence).