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. 2010 Jun 29;59(10):1355–1362. doi: 10.1136/gut.2010.207456

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© 2010, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

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Gp96 expression in Caco-2 cells. (A) Fold variation of gp96 mRNA levels in Caco-2 cells after 24 or 48 h of stimulation with tumour necrosis factor α (TNFα) or interferon γ (IFNγ) relative to that in non-treated cells (NT) using RT–PCR. gapdh (glyceraldehyde phosphate dehydrogenase) mRNA levels were measured as controls. Data are the mean±SEM of three separate experiments. (B and C). Western blot analysis showing expression levels of Gp96 and carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) by Caco-2 cells after 24 or 48 h of stimulation with 50 ng/ml TNFα or IFNγ (B) or after a 3 h infection period with adherent-invasive Escherichia coli (AIEC) strain LF82 (C) at a multiplicity of infection of 10 or 100. As loading control, labelling was performed using anti-β-actin polyclonal antibodies.