Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
| Strains | ||
| LF82 | E coli isolated from an ileal biopsy of a patient with CD | 6 |
| LF82-ΔompA | LF82 isogenic mutant with the ompA gene deleted | This study |
| K-12 MG1655 | Non-pathogenic E coli strain | Laboratory stock |
| Plasmids | ||
| pKOBEG | pBAD cloning vector harbouring the λ phage redγβα operon, chloramphenicolr | 27 |
| pBAD33 | E coli cloning vector, chloramphenicolr | 47 |
| pPBI13 | pBAD33 harbouring the 1.1 kb HindIII–SalI fragment with the entire ompA gene of strain LF82 | This study |
| pPBI14 | pBAD33 harbouring the 1.1 kb HindIII–SalI fragment with the entire ompA gene of strain MG1655 | This study |
| pSUPER.neo | Vector system for expression of small interfering RNA | Oligoengine |
| gp96 siRNA | pSUPER.neo harbouring the oligonucleotide specific to the gp96 mRNA | This study |
| Control siRNA | pSUPER.neo harbouring the oligonucleotide control | This study |