TABLE 4.
Primer sequences for amplification of szeF, szeN, and szeP superantigen genes
| Gene and primera | Sequence (5′ to 3′) |
|---|---|
| szeF | |
| F1 | ATC-CGA-ATG-TAT-ACT-GAC-GAA-AAG |
| F2 | TTG-CTA-GCA-GAT-GAC-AGG-AAG-ATA |
| F3 | TTT-TAT-CTT-GTG-GCT-TTC-GTT-A |
| F4 | CTA-TTT-CTT-GGG-CTG-TTA-CTA-T |
| szeN | |
| N1 | AAA-TGC-TCA-AAA-GTG-CGA-CAG-G |
| N2 | ATA-GAA-GGT-AGA-GCC-CCA-AGA-TAA-GAT-A |
| N3 | AAT-GTA-AGT-TTA-TCC-GAA-GAA |
| N4 | AAT-ATC-CAG-TTG-AGA-AAT-CC |
| szeP | |
| P1 | TGT-CGC-ACT-TTT-GAG-CAT-TTT-G |
| P2 | CTG-AGC-GAT-TTT-AAC-ATA-GTA-GTC |
| P3 | AAG-CGA-GTT-AAT-GGG-ATA-CGA-T |
| P4 | TCA-CCC-TTT-ACA-AAT-TTA-CCT-T |
a
Two sets of primers were designed for each gene: one pair to amplify the full-length gene (primers 1 and 2 [e.g., primers F1 and F2 for the szeF gene]) and another pair to amplify a shorter internal sequence (primers 3 and 4 [e.g., primers F3 and F4 for the szeF gene]).