FIG. 11.

Effect of a pharmacological inhibitor of NF-κB on CXCL8 secretion by bMEpC incubated with LTA or MDP. Bovine MEpC were preincubated for 1 h at 37°C with 15 μM NF-κB activation inhibitor (Calbiochem) before the addition of MAMPs (1 μg/ml). Cell culture supernatants were collected 8 h poststimulation, and CXCL8 concentrations were determined by ELISA. Results are median values (Q1 and Q3) from bMEpC of five cows *, reductions of CXCL8 concentrations by NF-κB inhibition are significant (P < 0.05 by Friedman test and Bonferroni's multiple comparisons).