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. 2010 Sep 8;17(11):1797–1809. doi: 10.1128/CVI.00268-10

FIG. 5.

FIG. 5.

Secretion of the chemokines CXCL8 and CXCL3 by bMEpC stimulated with LTA and MDP alone or in combination. (a) Dose-response of bMEpC from one cow to LTA and MDP. (b) Susceptibility of LTA to PAF-AH or proteinase K. Purified LTA (200 ng/ml) was used to stimulate bMEpC; alternatively, LTA (200 ng/ml) was pretreated with the LTA-inactivating enzyme PAF-acetyl-hydrolase (LTA-PAF-AH) or with lipoproteins inactivating proteinase K (LTA-Prot K). LTA (200 ng/ml) was added to the treated LTA to check whether PAF-AH treatment had generated inhibitors of CXCL8 secretion (LTA-PAF-AH or LTA). (c) Secretion of CXCL8 by MEpC (median values from cells of five cows, first quartile [Q1] and Q3) in response to LTA and MDP at 8 h and 24 h poststimulation. Shown is the secretion of CXCL3 in response to LTA and MDP at 1 μg/ml. (d) Secretion of CXCL3 in response to LTA and MDP at 0.1 μg/ml. MDP-DD, inactive isoform of muramyl-dipeptide (MurNAc-d-Ala-d-iso-Gln); MDP, active isoform (MurNAc-l-Ala-d-iso-Gln).