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. 2010 Sep 8;17(11):1797–1809. doi: 10.1128/CVI.00268-10

FIG. 7.

FIG. 7.

Production of TNF-α and IL-1β by cells stimulated with LTA or MDP. (a) bMEpC stimulated with LTA (1 μg/ml), MDP (1 μg/ml), or a combination of LTA plus MDP (1 μg/ml) responded with an increased level of expression of TNF-α mRNA (P < 0.05 by Kruskal-Wallis test). Shown are median values (Q1 and Q3) from bMEpC of five cows. (b) TNF-α in cell culture supernatants after 16 h of culture; results are from a representative experiment with either bMEpC or leukocytes. (c) IL-1β mRNA levels in response to LTA or MDP. Shown are median values (Q1 an Q3) from bMEpC of five cows. (d) IL-1β in cell culture supernatants after 16 h of culture; results are from the bMEpC of five cows or a representative experiment with either bMEpC or leukocytes. RE/18S, expression relative to 18S rRNA; PMA, phorbol myristate acetate; Mono, monocytes; PBMC, peripheral blood mononuclear cells stimulated with PMA and ionomycin.