FIG. 8.

Expression of transcripts of genes encoding PRR (NOD2, TLR2, and TLR6), peptide transporter (PEPT1), or PAF receptor by MEpC or bovine mammary tissue. RNA was extracted from bMEpC of two cows (cows 2006 and 1018), which were left unstimulated or were stimulated with LTA plus MDP (1 μg/ml each) for 8 h. RNA was also obtained from the mammary tissue of uninfected uninflamed quarters of two lactating cows (cows 5011 and 4019). RT-PCR was performed as described in Materials and Methods. GAPDH expression was used as a positive control.