TABLE 1.
Primers used in this study
| Gene | Oligonucleotide sequence (5′-3′)a | Amplicon size (bp) | Annealing temp (°C) | Annealing duration (s) | GenBank accession no. |
|---|---|---|---|---|---|
| 18S rRNA | CGGGGAGGTAGTGACGAAA (F) | 196 | 69 | 8 | AF176811 |
| CCGCTCCCAAGATCCAACTA (R) | |||||
| IL-1β | CTCTCACAGGAAATGAACCGA (F) | 152 | 67 | 4 | EU276067 |
| GCTGCAGGGTGGGCGTATCACC (R) | |||||
| TNF-α | TCTTCTCAAGCCTCAAGTAACAAGC (F) | 104 | 69 | 5 | EU276079 |
| CCATGAGGGCATTGGCATAC (R) | |||||
| IL-6 | TGCTGGTCTTCTGGAGTATC (F) | 153 | 62 | 5 | EU276071 |
| GTGGCTGGAGTGGTTATTAG (R) | |||||
| GAPDH | GGCATCGTGGAGGGACTTATG (F) | 186 | 62 | 30 | DQ403066 |
| GCCAGTGAGCTTCCCGTTGAG (R) | |||||
| NOD2 | CCCAGGGGCTCAGAACTAACA (F) | 238 | 62 | 30 | NM_001002889 |
| CCTTCATCCTGGACGTGGTTC (R) | |||||
| PAFR | CCTTTCGTGTGGACTCAGAGTTCCG (F) | 403 | 62 | 30 | NM_001040538 |
| ATCAGGGACAGAAGGATGCCACG (R) | |||||
| PEPT1/SLC15A1 | CTGGGCCTTGTTTGATCAGC (F) | 123 | 62 | 30 | NM_001099378 |
| ACGATCAGGATGGCGTTCAC (R) | |||||
| TLR2 | ACTGGGTGGAGAACCTCATGGTCC (F) | 307 | 62 | 30 | NM_174197 |
| ATCTTCCGCAGCTTACAGAAGC (R) | |||||
| TLR6 | CTCCGGGAGATAGTCACTTC (F) | 297 | 62 | 30 | NM_001001159 |
| GGCCCTGGATTCTATTATGG (R) |
a
F, forward; R, reverse.