FIG. 6.

MBA detection of IgG antibodies among giardiasis serum sets. The MBA was conducted as described in Materials and Methods using beads coated with rVSP1/GST through rVSP6/GST (V1 to V6), α-1 giardin (α-1), rCp17/GST (17), rCp27/GST (27), and GST alone (GST). Bound IgG antibodies were detected using a biotinylated mouse monoclonal anti-human IgG and R-phycoerythrin-labeled streptavidin. IgG antibody response distributions for the U.S. sporadic giardiasis confirmed case patient set (n = 14) (A), the BC giardiasis outbreak sample set (n = 70) (B), and the Canadian student giardiasis outbreak sample set (n = 21) (C) are shown. Boxes include values between the 25th and 75th percentiles, whiskers include values between the 10th and 90th percentile, and outliers are indicated by data points. The median values are indicated within the box by a horizontal line.