TABLE 2.

Effects of denaturing and reducing conditions on the serologic IgG antibody responses to Giardia VSPs^b

Pretreatment (Ta)	Blocking	Response to treatmentc
		VSP1	VSP2	VSP3	VSP4	VSP5	VSP6	GST
None	Normal	100c	88	101	77	101	83	1
1% SDS (RTd)	Normal	99	93	100	74	104	88	2
1% SDS (37)	Normal	96	77	99	24	105	79	2
1% SDS (65)	Normal	62	35	88	13	99	50	2
1% SDS (95)	Normal	28	47	79	19	79	34	1
None	10 mM DTT	1	4	2	1	73	6	2
None	50 mM DTT	2	4	2	1	21	4	2

^aT, pretreatment temperature (°C).

^bAll VSP protein fragments were assayed as GST fusion proteins.

^cResponses (OD₄₀₅) are expressed as a percentage of the value obtained for rVSP1/GST under normal assay conditions (set at 100%).

^dRT, room temperature.