TABLE 3.
Effects of denaturing and reducing conditions on the mouse IgG antibody response to rVSP1b
| Pretreatment (Ta) | Blocking | Response to treatment |
||||||
|---|---|---|---|---|---|---|---|---|
| VSP1 | VSP2 | VSP3 | VSP4 | VSP5 | VSP6 | GST | ||
| None | Normal | 100c | 2 | 42 | 20 | 0 | 1 | 1 |
| 0.5% SDS (95) | Normal | 63 | 1 | 37 | 8 | 0 | 2 | 0 |
| None | 50 mM DTT | 77 | 0 | 5 | 1 | 0 | 0 | 1 |
a
T, pretreatment temperature (°C).
b
All VSP protein fragments were assayed as GST fusion proteins.
c
Responses (OD405) are expressed as a percentage of the value obtained for rVSP1/GST under normal assay conditions (set at 100%).