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SPR analysis of SEB binding Fab fragment. SEB was covalently immobilized by amine coupling to the dextran matrix of a sensor chip CM3 to yield 1,200 resonance units. Followed by blocking with ethanol amine, serially diluted ab53981 (A), Fab fragment (B), and BSA (C) were introduced to the chip at a flow rate of 30 μg/min, and the responses were recorded.
