FIG. 7.

Characterization of scFv binding to SEB. (A) Purified scFv (1 μg) was subjected to SDS-PAGE and Coomassie blue stained. (B) Serially diluted scFv was poured into an SEB-coated (•) or an uncoated (○) polystyrene plate. After the plate was washed with buffer, scFv was detected by using HRP-labeled protein L. (C) Serially diluted scFv were introduced to sensorchip-immobilized SEB. (D) E. coli cell lysates containing N15-MBP, 1 μg of BSA, and 1 μg of SEB were separated by SDS-PAGE and subjected to Western blot analysis with scFv antibody for primary detection.