TABLE 1.
PCR primers used in this study
| Primer | DNA sequence (5′-3′)a |
|---|---|
| N1-15F | TTCTATTCTCACTCTGAGAGTCAACCAGATCCTAAACCAGAT |
| N1-15R | GGCCGAACCTCCACCACTCGATTTGTGCAACTCATCTGGTTTAGGATC |
| Acc65I Forward | ATTCCTTTAGTGGTACCTTTCTATTCTCACTCT |
| EagI Reverse | TTCAACAGTTTCGGCCGAACCTCCACC |
| His-tag F | GAGTCATTCTGCGGCCGCACATCATCATCATCATCACGCCGCATAGACTGTTGAAAGTTGTTT |
| His-tag R | GGCCAGTGAATTCTTATTAAGACTCCTTATTAC |
| pET Forward | GGAGATTTTCCATATGAAAAAATTATTATTCGC |
| pET Reverse | AAACAACTTTCGGATCCCTATGCGGCGTGATGATG |
| MBP-scFv Forward | CCTTTCTATGCGGCCCAGGGATCCATGGCCCAGGT |
| IA+IB | SAGGTGCAGCTKCTCGAGTCAGGACCTRGC |
| IIA | SAGGTYCAGCTGCTCGAGTCTGGASCTGAG |
| IIB | CAGGTCCARCTGCTCGAGYCTGGGGCTGAG |
| IIC | GAGGTTCAGCTGCTCGAGTCTGKGGCWGAG |
| 3A | GARGTGAAGGTGCTCGAGTCTGGRGGAGGC |
| 3B+3C | GARGTGAAGCTTCTCGAGTCTGGAGGWGGC |
| 3D | GARGTGCAGCTGCTCGAGGGKGGGGGAGGA |
| IgG1 | CGCGCGACTAGTACCACAATCCCTGGGCACAATTTT |
| κI | GCGCGCGAGCTCGACRTTGTGATGWCACAGTCTCCATCCTYC |
| κII | GCGCGCGAGCTCGATRTTKTGATGACCCARACTCCACTCTCC |
| κIII | GCGCGCGAGCTCGACATTGTGCTGACMCARTCTCCWGCTTCC |
| κIV | GCGCGCGAGCTCSAAAWTGTKCTCACCCAGTCTCCAGCAATC |
| κV | GCGCGCGAGCTCGAYATYCAGATGACMCAGWCTMCATCCTCC |
| κVI | GCGCGCGAGCTCCAAATTGTKCTCWCCCAGTCTCCAGCAATC |
| κ | GCGCGCTATCTAGAATTAACACTCATTCCTGTTGAAGCTCTT |
a
Restriction sites are indicated by underlining.