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. 2001 Mar 15;29(6):e32. doi: 10.1093/nar/29.6.e32

Figure 1.

Figure 1

Construction of TAR cloning vectors containing a different length of homology to the Tg.AC transgene. (A) The structure of the Tg.AC transgene. The transgene unit consists of a ζ-globin promoter fused to the v-Ha-ras structural gene with a terminal SV40 polyadenylation signal sequence (5,20). Nine different sizes of targeting sequence (from 800 to 20 bp), shown by filled bars, were selected from the 3′-terminal region of the transgene containing an SV40 polyadenylation signal sequence. (B) A scheme of a TAR vector. A set of TAR vectors with targeting sequences of different sizes (800, 600, 373, 284, 186, 118, 60, 40 and 20 bp) homologous to the 3′-end of the transgene was constructed. Each vector contains a 130 bp B1 repeat as a second targeting sequence. Vectors were linearized by SalI restriction endonuclease before transformation.