Table 3. TAR cloning of Tg.AC trangene by vectors containing different sizes of targeting sequences.
| TAR vector |
No. of transformants analyzeda |
No. of Tg.AC positive clones |
Yield (%) |
| SV800 | 200 | 3 | 1.50 |
| SV600 | 400 | 10 | 2.50 |
| SV373 | 1000 | 27 | 2.70 |
| SV284 | 200 | 4 | 2.00 |
| SV184 | 200 | 5 | 2.50 |
| SV118 | 800 | 19 | 2.38 |
| SV60 | 800 | 18 | 2.25 |
| SV40 | 800 | 4 | 0.50 |
| SV20 | 400 | 0 | 0 |
aTransformants were selected from several independent transformation experiments. The frequency of transformation varied from 296 to 976 His+ colonies in different TAR cloning experiments.