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. 2001 Mar 15;29(6):e32. doi: 10.1093/nar/29.6.e32

Table 3. TAR cloning of Tg.AC trangene by vectors containing different sizes of targeting sequences.

TAR vector
No. of transformants analyzeda
No. of Tg.AC positive clones
Yield (%)
SV800 200 3 1.50
SV600 400 10 2.50
SV373 1000 27 2.70
SV284 200 4 2.00
SV184 200 5 2.50
SV118 800 19 2.38
SV60 800 18 2.25
       
SV40 800 4 0.50
SV20 400 0 0

aTransformants were selected from several independent transformation experiments. The frequency of transformation varied from 296 to 976 His+ colonies in different TAR cloning experiments.