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. 2010 Sep 22;17(11):1763–1771. doi: 10.1128/CVI.00133-10

FIG. 3.

FIG. 3.

Polyfunctional profiles of Mtb72F-specific CD4+ T cells expressing any combination of immune markers among IFN-γ, IL-2, TNF-α, and CD40L. PBMCs from subjects vaccinated with 10 μg Mtb72F/AS02A (A) and 40 μg Mtb72F/AS02A (B) were obtained at prevaccination (day 0), 2 weeks after dose 2 (day 42), 2 weeks after dose 3 (day 70), prebooster (day 308), 1 month postbooster (day 338), and 1 year postbooster (day 644). PBMCs were evaluated using ICS and flow cytometry after short-term in vitro stimulation with 15-mer peptide pools spanning the Mtb32A and Mtb39A antigens. Data are represented in box-and-whiskers plots as the number of Mtb72F-specific CD4+ T cells expressing single markers and any combination of IFN-γ, IL-2, TNF-α, and/or CD40L per 106 CD4+ T cells.