TABLE 3.
Resistance selection with EFV in HIV-1 A, B, and C subtypes in the presence of 10% FBS under low-MOI conditionse
| Subtype | Expt | 1× EC95a |
2× EC95 |
5× EC95 |
10× EC95 |
50× EC95 |
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Tb | Mutation(s) | T | Mutation(s) | T | Mutation(s) | T | Mutation(s) | T | Mutation(s) | ||
| A | 1 | 34 | NMc | 12 | Y188(Y/C) | 10 | Y188(Y/C) | 8 | V106(V/M) | NAd | |
| F227(F/C) | F227(F/C) | ||||||||||
| A288(A/T) | A288(A/T) | ||||||||||
| 2 | 24 | D312E | 17 | D312E | 15 | D312E | 16 | D312E | 11 | D312E | |
| Q452L | Q452L | Q452L | Q452L | Q452L | |||||||
| L234(L/F) | L234F | L234F | |||||||||
| F77(F/Y) | F77Y | F77Y | |||||||||
| 3 | 30 | NM | 10 | V106(V/M) | 10 | V106(V/M) | 8 | V106M | 9 | V106M | |
| B | 1 | 11 | L100(L/I) | 9 | L100I | 13 | L100I | 11 | L100I | 28 | L100I |
| N348I | |||||||||||
| 2 | 10 | V179(V/D) | 5 | V179D | 7 | V179D | 8 | V179D | V179Df | ||
| L100(L/I) | L100I | L100If | |||||||||
| V108If | |||||||||||
| 3 | 13 | NM | 9 | K103N | 9 | K103N | 8 | K103N | 8 | K103N | |
| Y188(Y/C) | Y188C | ||||||||||
| C | 1 | 34 | G190A | 10 | G190A | 12 | G190A | 12 | G190A | 6 | G190A |
| L279F | L279F | L279F | L279F | L279F | |||||||
| V106(V/M) | V106M | V106M | |||||||||
| 2 | 34 | G190A | 10 | G190A | 12 | G190A | 12 | G190A | 12 | G190A | |
| L279F | L279F | L279F | L279F | L279F | |||||||
| V106M | V106M | ||||||||||
a
The EC95 of EFV with subtype B virus is 3.8 ± 1.4 nM (24).
b
T, time (in days).
c
NM, no mutation was detected.
d
NA, not available.
e
Amino acids in parentheses indicate a mixture of the wild-type residue and the emerging mutation.
f
Based on clonal sequencing, 14 out of 20 colonies contained L100I/V179D/V108I mutations, and 6 out of 20 clones had L100I/V179D mutations.