Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Strains and plasmids
| Strain/plasmid | Selection marker(s)a | Source/reference |
|---|---|---|
| M. smegmatis strains | ||
| SMR5 ΔrrnA ΔrrnB attB::pMIG-sacB-rrnB (parent) | Strr Genr | 16 |
| SMR5 ΔrrnB rrnA 2055A | Strr | 30 |
| SMR5 ΔrrnA ΔrrnB attB::pMIH-rrnB (wild type) | Strr HygBr | This study |
| SMR5 ΔrrnA ΔrrnB attB::pMIH-rrnBmutation | Strr HygBr | This study |
| Plasmids | ||
| pMIHΔint-rrnB | HygBr | 16 |
| pMIHΔint-rrnBmutation | HygBr | This study |
Str, streptomycin; Gen, gentamicin; HygB, hygromycin B.
