TABLE 2.
DNA oligonucleotide primers
| Name | Sequence (5′ to 3′)c | Positionsa |
|---|---|---|
| OPF3 | GAGTGATCTACCCATGGCCAG | 798-818 |
| OPF4 | GACCAAGGGTTCCTGGGCCAG | 1421-1441 |
| OPF5 | GGTTTGTGTAGGATAGGTGGG | 2331-2351 |
| OPR3 | GTGCCCCTGGTGGGACAACTG | 2919-2939 |
| OPR4 | CGAGTCGCGACGTACGTTCG | Noneb |
| OPR5 | TCGAGCTCGGTACCTACGTAC | Noneb |
| OPR7 | GGTCGAGAAGTAACAGGG | Noneb |
| SP1F | CCTGCACGAATGGCGTAACG | 2188-2207 |
| SP2F | GAGACGGACATGTCGAGCAG | 2578-2597 |
| G2032AF | CTACGAGTAAAAATGCTCGTTACG | 2248-2271 |
| G2032AR | AACGAGCATTTTTACTCGTAGTG | 2246-2268 |
| G2032CF | CTACGAGTAAACATGCTCGTTACG | 2248-2271 |
| G2032CR | AACGAGCATGTTTACTCGTAGTG | 2246-2268 |
| A2058GF | GCAGGACGAGAAGACCCCGGGAC | 2276-2298 |
| A2058GR | CCGGGGTCTTCTCGTCCTGCCGC | 2273-2295 |
| A2453UF | CGGGGATAACTGGCTGATCTTCC | 2670-2692 |
| A2453UR | AAGATCAGCCAGTTATCCCCGGG | 2668-2690 |
| C2499AF | GTTTGGCACATCGATGTCGGCT | 2717-2738 |
| C2499AR | CGACATCGATGTGCCAAACCATC | 2713-2735 |
| C2499UF | GTTTGGCACTTCGATGTCGGCT | 2717-2738 |
| C2499UR | CGACATCGAAGTGCCAAACCATC | 2713-2735 |
| A2503GF | TGGCACCTCGGTGTCGGCTCGTC | 2720-2742 |
| A2503GR | CGAGCCGACACCGAGGTGCCAAAC | 2717-2740 |
| U2504GF | CACCTCGAGGTCGGCTCGTCG | 2723-2743 |
| U2504GR | GAGCCGACCTCGAGGTGCCAAAC | 2717-2739 |
| G2505AF | GGCACCTCGATATCGGCTCGTCGC | 2721-2744 |
| G2505AR | CGAGCCGATATCGAGGTGCCAAAC | 2717-2740 |
| C2571GF | ATTAAAGCGGGACGCGAGCTGGG | 2788-2810 |
| C2571GR | CAGCTCGCGTCCCGCTTTAATGGG | 2785-2808 |
| A2572UF | TAAAGCGGCTCGCGAGCTGGGT | 2790-2811 |
| A2572UR | CAGCTCGCGAGCCGCTTTAATGG | 2786-2808 |
| G2576UF | AGCGGCACGCTAGCTGGGTTTAG | 2793-2815 |
| G2576UR | AACCCAGCTAGCGTGCCGCTTTA | 2790-2812 |
| C2612AF | CAGTTCGGTCTATATCCGCCGC | 2828-2849 |
| C2612AR | GCGGCGGATATAGACCGAACTGTC | 2826-2849 |
a
Positions of M. smegmatis 23S rRNA gene complementarity are given.
b
Primers are complementary to pMIHΔint-rrnB at sites downstream of rrnB and the MunI site.
c
Mutated nucleotides are underlined in the sequences of primers used for site-directed mutagenesis.