TABLE 1.

Primer sequences used to confirm AMEs present in AG-R clinical isolates

AME	Forward primer	Reverse primer
Gram-negative AMEs
AAC(3)-Ia	ACAAAGTTAGGTGGCTCAAGTATGGGCATC	TCACCGTAATCTGCTTGCACGTAGATCAC
AAC(3)-IIa	GCCGACTGGCACTGTGATGGGATAC	TGCAATGCGGTAACGGAGTTTAGCG
AAC(3)-IVa	CTCGAAGATGGGCCACTTGGACTGATC	AACTCGGCAAGATGCAGCGTCGTG
AAC(6′)-Ib/II	GTGACCAACAGCAACGATTCCGTCAC	GTGACCTCGGGATCATTGAACAGCAAC
ANT(2″)-Ia	GTGTAACACGCAAGCACGATGATATTGATCTG	CGAGCCTGTAGGACTCTATGTGCTTTGTAGG
APH(3)-VIa	CACATACAGTGTCTCTCGTGAAGCGAAAATG	CGTGATATCGCCATGAGAAAAAACCAATC
Gram-positive AMEs
AAC(6′)-Im	CGCCCGATGAATGAGGATGAT	TCCTGCCTTCTGATATGCTCG
ANT(4′)-Ia	ATGGACAACCGGTGAGTGGAA	CGTTCTGTCCACTCCTGAATC
APH(2″)-Ia + AAC(6′)-Ie	TTGAAATAATCGGTAGTGGTTATGATAGTGTGGCA	CCCTCAAAAACTGTTGTTGCATTTAGTCTTTCC
APH(2″)-Ib	GTCCGGCGATGATAGTGATAC	CGGCGCCTTATGCTGATAGTA
APH(2″)-Ic	GAGTCAACAAGGTGCAGACGA	CTCGCCTCTATAAGCCATCAC
APH(2″)-Id	CAGGAGAAGGCAATGACTGT	CGGGATCAGAAATAGCTGCA
APH(3′)-III	TGACGGACAGCCGGTATAAAG	GGCGCAGAAGGGAATGTCAT