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A-NO2−-mediated killing of biofilm bacteria. Biofilms were grown for 1 day under aerobic conditions in MH medium. Following A-NO2− addition, biofilms were maintained under anaerobic conditions for an additional 2 days. The results represent CFU per ml of biofilms at different NaNO2 concentrations (means ± standard errors of means are shown; n = 3). (A) P. aeruginosa FRD1; (B) P. aeruginosa PAO1; (C) S. aureus USA300; (D) B. cepacia.
