FIG. 3.

Effects of sulfated K5 derivatives on cell-to-cell spread of HCMV. (A) HFFs were infected with HCMV AD169 (MOI, 0.003 PFU/cell) in the absence of compounds (HCMV) or not infected (Mock). Either K5-N,OS(H) or K5-NS was then added at different concentrations, and cells were incubated for 5 days at 37°C. Viral cell-to-cell spread was assessed by indirect immunofluorescence with an anti-IEA MAb. (B) Fifteen representative infected foci per concentration were analyzed with ImageJ software to determine the viral plaques size in the presence of the test compounds. The data shown represent the means ± the SD. The asterisks denote a statistically significant difference (*, P < 0.05; **, P < 0.001).