TABLE 2.
Primers used in this study
| Primer | Sequence (5′-3′)a | PCR product length (bp) | Use |
|---|---|---|---|
| AG-FB | GGGATCCCCTGCACAACGACAGGGTGGACATACG | 1,250 | Cloning of PA4393 (ampG) |
| AG-RHB | GAAGCTTTCAAGATCTGTGCTGCTCGGCGTTCTGGTGTC | ||
| AGh1-FR | GGAATTCGTCACCGGAGACCACCATGCTTGAG | 1,882 | Cloning of PA4218 (ampGh1) |
| AGh1-RH | GAAGCTTAGGTGGAACGGCCACGCTAGCAACA | ||
| AG-FUR | GGAATTCGAACCAGTGTTCGTCGAAGAAGCGATC | 988 | PA4393 upstream fragment |
| AG-RUB | GGGATCCGCGCACTCTAACCGCTCTACTTCGCTG | ||
| AG-FDB | GGGATCCGGCGAGAATGAAAAAGGCCGGCATTCG | 1,012 | PA4393 downstream fragment |
| AG-RDH | GAAGCTTCCAGGCCCGGAAACGTCGCCCCACGG | ||
| AGh1-FUR | GGAATTCTGGCGCTGTTCGACCTGCATGGCCTG | 1,023 | PA4218 upstream fragment |
| AGh1-RUB | GGGATCCGCTCAAGCATGGTGGTCTCCGGTGAC | ||
| AGh1-FDB | GGGATCCGGCGCTGCCGATAGGCGCGCAGCGCC | 1,166 | PA4218 downstream fragment |
| AGh1-RDH | GAAGCTTCGCGGCGAACTGCAGATGATCCTGCTC |
a
Underlined are restriction endonuclease sites. Primer sequences are based on the published PAO1 genome sequence (46).