TABLE 3.
MICs and specific activities for AmpC in the indicated strains
| Strain | MIC (μg/ml) |
β-Lactamase activitya |
|||
|---|---|---|---|---|---|
| Ampicillin | Cefotaxime | Ceftazidime | Noninduced | Inducedb | |
| PAO1 | 256 | 16 | 1 | 4 ± 0.5 | 879 ± 54 |
| PAK | 512 | 8 | 1 | 6 ± 0.3 | 1,219 ± 65 |
| PAO1ΔDDh1Dh2 | 1,024 | 512 | 32 | 3,380 ± 462 | 3,438 ± 367 |
| PAO1ΔG | 32 | 8 | 1 | 3 ± 0.3 | 0 |
| PAO1ΔGh1 | 256 | 16 | 1 | − | − |
| PAO1ΔGGh1 | 32 | 8 | 1 | − | − |
| PAO1ΔG/pUCP24 | 32 | 8 | 1 | − | − |
| PAO1ΔG/pZY0901 | 1,024 | 256 | 16 | 52 ± 4 | 1,303 ± 87 |
| PAO1ΔG/pZY0908 | 32 | 8 | 1 | − | − |
| PAKΔG | 32 | 8 | 1 | 2 ± 0.2 | 0 |
| PAKΔGh1 | 512 | 8 | 1 | − | − |
| PAKΔGGh1 | 32 | 8 | 1 | − | − |
| PAKΔG/pUCP24 | 32 | 4 | 1 | − | − |
| PAKΔG/pZY0901 | 512 | 512 | 32 | 1,095 ± 65 | 2,573 ± 239 |
| PAO1ΔDDh2Dh3G | 16 | 4 | 1 | 3 ± 0.1 | 0 |
| PAO1ΔDDh2Dh3Gh1 | 1,024 | 256 | 32 | − | − |
| PAO1ΔDDh2Dh3G/pUCP24 | 16 | 4 | 1 | − | − |
| PAO1ΔDDh2Dh3G/pZY0901 | 1,024 | 512 | 32 | 6,656 ± 326 | 8,130 ± 779 |
| SNO301-1/pEC1C/pUCP26 | 16 | <2 | − | 43 ± 2 | 38 ± 2 |
| SNO301-1/pEC1C/pGKS273-5 | 1,024 | 64 | − | 1530 ± 80 | 3600 ± 90 |
| SNO301-1/pEC1C/pZY0901 | 1,024 | 32 | − | 161 ± 8 | 3250 ± 110 |
a
Nanomoles of nitrocefin hydrolyzed per minute per milligram of protein. −, not tested.
b
Induction was carried out with 50 μg of cefoxitin per ml for 2 h.