Target sites of Tn5397 within C. difficile 630 (CD630), C. difficile R20291, and E. faecalis JH2-2. Tn5397 sequence is shown in boldface. TS, target site in the genome; LE, left end of Tn5397; RE, right end of Tn5397; CI, circular intermediate of Tn5397; TS2, the second target site in C. difficile R20291. All sequences are derived from PCR amplicons.