FIG. 1.

Effect of increasing concentrations of CMX001 on BKV load and expression of BKV proteins. (a) RPTEC supernatants were harvested 72 hpi, i.e., 70 h after the start of treatment with indicated CMX001 concentrations, and BKV load was measured by qPCR. The DNA load in untreated cells (1.19 × 10⁹ genome equivalent [Geq]/ml) was set as 100%. Determinations were in triplicate. The mean values are shown, and the error bars represent standard deviations. (b) Indirect immunofluorescence of BKV-infected RPTECs either untreated or treated with the indicated CMX001 concentrations. The cells were methanol fixed 72 hpi and stained using as primary antibodies polyclonal rabbit anti-agno serum (green) for the visualization of the late agno and the SV40 LT-ag monoclonal Pab416 for the visualization of early LT-ag (red). Cell nuclei (blue) were stained with Drac 5.The pictures are taken with the 10× objective.