TABLE 3.
Activity of BI 201335, telaprevir, and boceprevir in subgenomic replicon, JFH1 viral replication, and cytotoxicity assays in Huh-7 cells
| Inhibitor | EC50 (nM) against HCV genotypea: |
CC50 (nM)b | |||
|---|---|---|---|---|---|
| 1a | 1b | 2a replicon | 2a virus | ||
| BI 201335 | 6.5 ± 0.9 | 3.1 ± 1.2 | 50 ± 7 | 43 ± 27 | 30,000 |
| Telaprevir | 700 ± 50 | 540 ± 30 | ND | ND | 37,000 |
| Boceprevir | 550 ± 210 | 520 ± 220 | ND | ND | >70,000 |
a
Fifty percent effective concentrations (EC50) values shown are means ± standard deviations from at least three independent experiments. ND, not determined.
b
The cytotoxic concentration of drug that reduced the viable cell number by 50% (CC50) was determined by adding MTT reagent (see Materials and Methods.