FIG. 1.

Schematic diagram depicting a restriction map of a 5.6-kb HindIII fragment of E. faecium JS79 DNA containing two streptogramin A resistance genes and a transposase gene. Bold arrows reflect the orientations of vgaD and vatG and the transposase (IS); black boxes reflect functional domains within the resistance gene translation product. There are two ABC transporter 2 domains, the N-terminal ATP binding domain and the C-terminal ATP binding domain, in the ABC transporter encoded by vgaD and an LβH domain in the streptogramin A acetyltransferase encoded by vatG. Filled arrows indicate primer sequences designed for the vgaD and vatG genes.