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RT-PCR analysis of the vca0421 transcript levels. Lane M, 100-bp DNA ladder marker; lane 1, wild-type strain; lane 2, CIP-resistant mutant CIP0.25-1; lane 3, mTn-321. The gyrB gene was used as an internal control. The relative expression level of the vca0421 transcript was calculated as the ratio of the RT-PCR product of the vca0421 gene to that of the gyrB gene (values shown at bottom).
