TABLE 1.
Comparison of results from three methods for screening and confirmation of C. botulinum serotypes A, B, E, and Fa
| Sample | Original enriched cultures |
Selectively enriched cultures |
|||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mouse bioassay result | ELISA results |
Real-time PCR results |
ELISA results |
Real-time PCR results |
|||||||||||||
| A | B | E | F | A | B | E | F | A | B | E | F | A | B | E | F | ||
| Positive control | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
| Negative control | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − |
| CMM samples | |||||||||||||||||
| Sample 4 | + | + | − | − | − | + | + | − | − | − | − | − | − | + | + | − | − |
| Sample 8 | + | + | − | − | − | + | + | − | − | + | − | − | + | + | + | − | − |
| Sample 10 | − | + | − | − | − | + | + | − | − | − | − | − | − | + | + | − | − |
| Sample 12 | + | + | − | − | − | + | + | − | − | + | − | − | − | + | + | − | − |
| Sample 14 | + | + | − | − | − | + | + | − | − | + | − | − | + | + | + | − | − |
| Sample 16 | + | + | − | − | + | + | + | − | + | + | − | − | + | + | + | − | − |
| TPGY samples | |||||||||||||||||
| Sample 4 | + | + | + | − | − | + | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| Sample 8 | + | ND | ND | ND | ND | + | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| Sample 10 | + | + | − | − | − | + | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| Sample 12 | + | + | − | − | − | + | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| Sample 14 | + | + | − | − | − | + | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| Sample 16 | + | + | − | − | − | + | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
a
Original enriched cultures used water samples enriched in CMM or TPGY; selectively enriched cultures were enriched on BSM solid medium. +, positive; −, negative; ND, data not determined for these samples.