FIG. 2.

(A) Physical maps of the BamHI-ClaI 4.1-kb region of pRB41. Abbreviations are for the endonuclease recognition sites: C, ClaI; B, BamHI; Ps, PstI; E, EcoRI. The numbers indicate kb. (B) Thick horizontal arrows indicate the predicted ORFs and the direction of ORF transcription. The thin arrow indicates the potential promoter region. The vertical lines with circular heads on the ORF map show the points of transposon insertion. The circular heads indicate Tn5, and the numbers (1, 2, 6, 7, and 9) are the same as those of Tn5 mutants shown in Fig. 1. (C) The horizontal lines under the map represent plasmids derived from the cloned fosmid library DNA fragments listed on the left. The dotted vertical lines represent the ends of the amplified PCR fragment of pRB41 used to clone the bacteriocin determinant. Carocin D activity is shown, based on results in panel D. +++, full activity; +, low activity; −, no activity. (D) Antibacterial activity expressed in E. coli DH5α for each subclone.