Strains |
|
|
E. coli
|
|
|
EPI300-T1R
|
F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ−rpsL nupG trfA dhfr
|
Epicentre |
EC100 pir+
|
F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ−rpsL nupG trfA dhfr
|
Epicentre |
DH5α |
F− φ80dlacZΔM15 endA41 recAl hsdR17(rK− mK−) supE44 thi-1 gyrA96
|
BRL |
BL21 |
hsdS gal (λcIts857 ind1 Sam7 nin5 lacUV-T7 gene 1) |
Novagen |
P. carotovorum subsp. carotovorum
|
|
|
Pcc3 |
Carocin D−, sensitive to carocin D, Rifr
|
17 |
Pcc21 |
Carocin D+, Rifr
|
17 |
Pcc21DK1,2,6,7,9 |
Pcc21; caroDK::Tn5, carocin D−, Rifr Kanr
|
This study |
Plasmids |
|
|
pBluescript II KS(+) |
AmprlacZ, cloning vector |
Stratagene |
pGEM-T Easy |
TA-cloning vector; Ampr
|
Promega |
pLUG-multi |
TA-cloning vector; Ampr
|
Intron |
pGFPuv |
Ampr, lac promoter |
Clontech |
pDSK519 |
Cloning vector; Kanr
|
8 |
pCC1FOS |
Fosmid vector; Chlr
|
Epicentre |
pRF6-A4 |
Fosmid library, pCC1FOS with 35-kb genomic DNA fragment |
This study |
pRB41 |
pBluescript II KS(+) with ClaI/BamHI 4.1-kb fragment |
This study |
pRG3431 |
pGEM-T Easy with 221 to ∼3,651 bp of ClaI/BamHI 4.1-kb fragment |
This study |
pRG2878 |
pGEM-T Easy with 385 to ∼3,263 bp of ClaI/BamHI 4.1-kb fragment |
This study |
pRG2808 |
pGEM-T Easy with 221 to ∼3,028 bp of ClaI/BamHI 4.1-kb fragment |
This study |
pRL1208 |
pLUG-multi with 1,821 to ∼3,028 bp of ClaI/BamHI 4.1-kb fragment |
This study |
pRG369 |
pGEM-T Easy with 2,895 to ∼3,262 bp of ClaI/BamHI 4.1-kb fragment |
This study |
pRP369 |
pGFPuv with HindIII/EcoRI 0.4-kb fragment of pRG369 |
This study |
pRD2808 |
pDSK519 with SalI/BamHI 2.9-kb fragment of pRG2808 |
This study |
Oligonucleotides |
|
|
caro-F-1762 |
5′-GAA AAC GCA GGC GGA AAC AGG TC-3′ |
This study |
caro-F-1926 |
5′-TTT AGC CAG ATC AGA GAA GTC-3′ |
This study |
caro-F-3362 |
5′-TTG CCG AAG AAA AAG AGA TAA-3′ |
This study |
caro-F-4436 |
5′-AAC CGT TGT ACA CTG ATG CT-3′ |
This study |
caro-R-3263 |
5′-AGC GCG TAT TAA TTA AGA ACA CA-3′ |
This study |
caro-R-4569 |
5′-GCA AAA ACG GTA AGC ACA TCT-3′ |
This study |
caro-R-4803 |
5′-GCG CGT ATT AAT TAA GAA CAC A-3′ |
This study |
caro-R-5192 |
5′-CGG CGT CAG CGT GGT GGT ATC-3′ |
This study |
DK3751 |
5′-GTA GCG GGC AGT TAT GAC AAA AAT-3′ |
This study |
DK4121 |
5′-ATC GGA CCG CCT GCC TTA TC-3′ |
This study |
DI4578 |
5′-CAG ATT ATA AAC AAT TGG ATA GAC-3′ |
This study |
DI4723 |
5′-CAC CCA ATT AGT CCC TTT TAC-3′ |
This study |
DKI3744 |
5′-CAG AGG TGT AGC GGG CAG TTA TGA-3′ |
This study |
DKI4730 |
5′-GCC ACC CAA TTA GTC CCT TTT ACA-3′ |
This study |