FIG. 1.

(A) Alignment of the regions showing differences between the β-fructofuranosidase from S. occidentalis described here (Ffase) and that previously reported (P24133). The differences are indicated in boldface. The Glu230 involved in sucrose hydrolysis, which is not present in P24133, is marked with an asterisk, and the Ser196 modified to Leu196 in S. cerevisiae is underlined. The superscript numbers indicate the amino acid positions. (B) Ffase was purified from S. occidentalis and digested with trypsin. The sequence of the peptide of 2,005 m/z identified by MALDI-TOF and generated by microspray-ion trap MS analysis is shown.